Host cell protein – ELISA assays
Host cell protein (HCP) ELISA based quantification In addition to DNA left overs from the upstream process, also proteins originating from cell line pose a big safety concern. Currently ELISA-based
Host cell DNA and pDNA quantity
Application of commercial qPCR assay (including high throughput DNA extraction) Custom designed protocol
Viral vector quantity
Determination with digital PCR (BioRad QX200 system) New assay development Evaluation and optimisation of established qPCR assays and/or transfer to ddPCR format
Sample preparation procedure
DNA/RNA extraction protocol development and qualification Evaluation of different commercial kits and approaches